Name: Puromycin Dihydrochloride
Brand: Selleck Chemicals
SKU: S7417
Rating: 5 (210 reviews)

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Controllo Qualità

Lotto: Purezza: 99.89%

99.89

Prodotti spesso usati con Puromycin Dihydrochloride

Blasticidin S Hydrochloride

It and Blasticidin S inhibit the proliferation and cell viability of metastatic castration-resistant prostate cancer (mCRPC) cells PC3/DU145.

Melatonin

Melatonin potentiates the apoptosis induced by this compound with caspase-3 and AMPK activation in HL-60 cells.

Target correlati	Integrase Bacterial Antibiotics Anti-infection Fungal Antiviral COVID-19 Parasite Reverse Transcriptase HIV
Altro Antineoplastic and Immunosuppressive Antibiotics Inibitori	Staurosporine (STS) Cyclosporin A Oligomycin A (MCH 32) Nigericin sodium salt Geldanamycin (NSC 122750) Honokiol Streptozotocin (STZ) Sodium Monensin (NSC 343257) Cephalomannine Pirarubicin

Coltura cellulare, trattamento e concentrazione di lavoro

Linee cellulari	Tipo di saggio	Tempo di incubazione	Descrizione dellattività	PMID
K562	Growth inhibition assay	72 h	Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay, IC50=26.83 μM.	18313307
HepG2	Growth inhibition assay	72 h	Growth inhibition of human HepG2 cells after 72 hrs by MTT assay, IC50=0.23 μM.	18313307
K562	Growth inhibition assay	72 h	Growth inhibition of human K562 cells after 72 hrs by SRB assay, IC50=0.22 μM.	18313307
HepG2	Growth inhibition assay	72 h	Growth inhibition of human HepG2 cells after 72 hrs by SRB assay, IC50=0.21 μM.	18512984
Huh7	Cytotoxicity assay		NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7), CC50=6.63 μM.	18579783
HEK239	Cytotoxicity assay	72 h	Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay, IC50=0.42 μM.	20558060
J774A1	Cytotoxicity assay		Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay, IC50=10 μM.	22934636
KB3-1	Growth inhibition assay	72 hrs	Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay, IC50 = 0.21 μM.	18313307
HepG2/Dox	Growth inhibition assay	72 hrs	Growth inhibition of multidrug resistant human HepG2/Dox cells after 72 hrs by SRB assay, IC50 = 20.2 μM.	18512984
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis, IC50 = 0.361 μM.	26651537
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay, IC50 = 0.3505 μM.	27060763
A549	Cytotoxicity assay		Cytotoxicity against human A549 cells, IC50 = 0.6 μM.	27089214
PC3	Cytotoxicity assay		Cytotoxicity against human PC3 cells, IC50 = 0.7 μM.	27089214
HEK293	Cytotoxicity assay		Cytotoxicity against HEK293 cells by alamar blue assay, IC50 = 0.00035 μM.	27212070
HEK293	Growth inhibition assay	72 hrs	Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay, IC50 = 0.36 μM.	28001067
HEK293	Growth inhibition assay		Growth inhibition of HEK293 cells, IC50 = 0.399 μM.	28400231
SU-DHL10	Cytotoxicity assay	72 hrs	Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.11 μM.	28523103
MIAPaCa2	Cytotoxicity assay	72 hrs	Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.2 μM.	28523103
MDA-MB-231	Cytotoxicity assay	72 hrs	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.48 μM.	28523103
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay, IC50 = 0.46 μM.	28774427
HL60	Antiparasitic assay	72 hrs	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay, EC50 = 0.055 μM.	28803047
MOLT4	Antiparasitic assay	72 hrs	Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay, EC50 = 0.17 μM.	28803047
Vero	Cytotoxicity assay	72 hrs	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay, CC50 = 2.8 μM.	28803047
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay, IC50 = 0.45 μM.	29236492
HEK293T	Cytotoxicity assay	72 hrs	Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.42 μM.	29469575
HCT8	Antiparasitic assay	48 hrs	Antiparasitic activity against Cryptosporidium parvum BGF infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.55 μM.	29469575
HCT8	Antiparasitic assay	48 hrs	Antiparasitic activity against Cryptosporidium parvum SPL infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.6 μM.	29469575
HepG2	Cytotoxicity assay	72 hrs	Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.83 μM.	29469575
HCT8	Cytotoxicity assay		Cytotoxicity against human HCT8 cells, EC50 = 6.1 μM.	29469575
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay, IC50 = 0.46 μM.	29533611
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay, IC50 = 0.4 μM.	29969262
A549	Cytotoxicity assay	48 hrs	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM.	30418763
PC3	Cytotoxicity assay	48 hrs	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM.	30418763
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Informazioni chimiche, conservazione e stabilità

Peso molecolare	544.43	Formula	C₂₂H₂₉N₇O₅.2HCl	Conservazione (Dalla data di ricezione)	3 anni-20°Cpolvere
N. CAS	58-58-2	Scarica SDF		Conservazione delle soluzioni stock	1 anno-80°Cin solvente 1 mese-20°Cin solvente
Sinonimi	CL13900 2HCl			Smiles	CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl

Solubilità

In vitro
Lotto:

DMSO : 100 mg/mL (183.67 mM)
(Il DMSO contaminato da umidità può ridurre la solubilità. Utilizzare DMSO fresco e anidro.)

Water : 100 mg/mL

Ethanol : Insoluble

Calcolatore di Molarità

Massa	Concentrazione	Volume	Peso molecolare
=	×	×

Calcolatore di Diluizione Calcolatore del Peso Molecolare

In vivo Lotto:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

Calcolatore di formulazione in vivo (Soluzione chiara)

Passo 1: Inserire le informazioni di seguito (Consigliato: Un animale aggiuntivo per tenere conto della perdita durante lesperimento)

Dosaggio: mg/kg Peso medio degli animali: g Volume di dosaggio per animale:μL Numero di animali:

Passo 2: Inserire la formulazione in vivo (Questo è solo il calcolatore, non la formulazione. Contattateci prima se non cè una formulazione in vivo nella sezione Solubilità.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Risultati del calcolo:

Concentrazione di lavoro: mg/ml;

Metodo per preparare il liquido master di DMSO: mg farmaco predissolto in μL DMSO ( Concentrazione del liquido master mg/mL, Vi preghiamo di contattarci prima se la concentrazione supera la solubilità del DMSO del lotto del farmaco. )

Metodo per preparare la formulazione in vivo: Prendere μL DMSO liquido master, quindi aggiungereμL PEG300, mescolare e chiarire, quindi aggiungereμL Tween 80, mescolare e chiarire, quindi aggiungere μL ddH₂O, mescolare e chiarire.

Metodo per preparare la formulazione in vivo: Prendere μL DMSO liquido master, quindi aggiungere μL Olio di mais, mescolare e chiarire.

Nota: 1. Si prega di assicurarsi che il liquido sia limpido prima di aggiungere il solvente successivo.
2. Assicurarsi di aggiungere il/i solvente/i in ordine. È necessario assicurarsi che la soluzione ottenuta, nellaggiunta precedente, sia una soluzione limpida prima di procedere allaggiunta del solvente successivo. Metodi fisici come il vortex, gli ultrasuoni o il bagno dacqua calda possono essere utilizzati per facilitare la dissoluzione.

Meccanismo dazione

In vitro	L'Antibiotics Puromycin Dihydrochloride è prodotta dall'actinomicete, Streptomyces alboniger, ed è stata utilizzata come strumento per studiare la sintesi proteica in numerosi sistemi. Puromycin Dihydrochloride può essere utilizzata per la selezione di cellule ricombinanti da cellule non coltivate.
In vivo	La Puromycin (aminonucleoside) inibisce la sintesi proteica portando a proteinuria e glomerulosclerosi/nefrosi.
Riferimenti	[1] https://pubmed.ncbi.nlm.nih.gov/14211122/ [2] https://pubmed.ncbi.nlm.nih.gov/15385422/ [3] https://pubmed.ncbi.nlm.nih.gov/28971619/ [4] https://pubmed.ncbi.nlm.nih.gov/3953770/ [5] https://pubmed.ncbi.nlm.nih.gov/32435426/ [6] https://pubmed.ncbi.nlm.nih.gov/11397778/

Applicazioni

Metodi	Biomarcatori	Immagini	PMID
Western blot	Cyclin D1 / CDK4 PARP / Bcl-xL / Bcl-2 / p-Akt / Akt p53 / p21 / c-Myc		31022952
Growth inhibition assay	Cell viability		31022952

Supporto tecnico

Istruzioni per la manipolazione

Tel: +1-832-582-8158 Ext:3

Per qualsiasi altra domanda, si prega di lasciare un messaggio.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Puromycin Dihydrochloride Inibitore della sintesi proteica

Struttura chimica

Peso molecolare: 544.43