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Akti-1/2 Akt inibitore
N. Cat.S7776
Struttura chimica
Peso molecolare: 551.64
Vai a
Controllo Qualità
Lotto:
Purezza:
99.11%
99.11
| Target correlati | PI3K mTOR GSK-3 ATM/ATR DNA-PK AMPK PDPK1 PTEN PP2A PDK |
|---|---|
| Altro Akt Inibitori | SC79 AZD5363 (Capivasertib) MK-2206 Dihydrochloride Ipatasertib (GDC-0068) Perifosine GSK690693 Triciribine (API-2) Afuresertib (GSK2110183) CCT128930 A-674563 HCl |
Coltura cellulare, trattamento e concentrazione di lavoro
| Linee cellulari | Tipo di saggio | Concentrazione | Tempo di incubazione | Formulazione | Descrizione dellattività | PMID |
|---|---|---|---|---|---|---|
| NCI-H1563 cell | Growth inhibition assay | Inhibition of human NCI-H1563 cell growth in a cell viability assay, IC50=0.54246 μM | ||||
| NCI-H1618 cel | Growth inhibition assay | Inhibition of human NCI-H1618 cell growth in a cell viability assay, IC50=21.9982 μM | ||||
| NCI-H1623 cell | Growth inhibition assay | Inhibition of human NCI-H1623 cell growth in a cell viability assay, IC50=15.0596 μM | ||||
| NCI-H1648 cell | Growth inhibition assay | Inhibition of human NCI-H1648 cell growth in a cell viability assay, IC50=5.7551 μM | ||||
| NCI-H1650 cell | Growth inhibition assay | Inhibition of human NCI-H1650 cell growth in a cell viability assay, IC50=11.5134 μM | ||||
| NCI-H1651 cell | Growth inhibition assay | Inhibition of human NCI-H1651 cell growth in a cell viability assay, IC50=16.4338 μM | ||||
| NCI-H1703 cell | Growth inhibition assay | Inhibition of human NCI-H1703 cell growth in a cell viability assay, IC50=15.577 μM | ||||
| NCI-H1734 cell | Growth inhibition assay | Inhibition of human NCI-H1734 cell growth in a cell viability assay, IC50=10.7788 μM | ||||
| NCI-H1755 cell | Growth inhibition assay | Inhibition of human NCI-H1755 cell growth in a cell viability assay, IC50=1.05531 μM | ||||
| NCI-H1770 cell | Growth inhibition assay | Inhibition of human NCI-H1770 cell growth in a cell viability assay, IC50=34.1793 μM | ||||
| NCI-H1792 cell | Growth inhibition assay | Inhibition of human NCI-H1792 cell growth in a cell viability assay, IC50=29.8379 μM | ||||
| NCI-H1963 cell | Growth inhibition assay | Inhibition of human NCI-H1963 cell growth in a cell viability assay, IC50=19.7494 μM | ||||
| NCI-H1993 cell | Growth inhibition assay | Inhibition of human NCI-H1993 cell growth in a cell viability assay, IC50=22.615 μM | ||||
| NCI-H2009 cell | Growth inhibition assay | Inhibition of human NCI-H2009 cell growth in a cell viability assay, IC50=7.39019 μM | ||||
| NCI-H2030 cell | Growth inhibition assay | Inhibition of human NCI-H2030 cell growth in a cell viability assay, IC50=1.94483 μM | ||||
| NCI-H2122 cell | Growth inhibition assay | Inhibition of human NCI-H2122 cell growth in a cell viability assay, IC50=10.1385 μM | ||||
| NCI-H2126 cell | Growth inhibition assay | Inhibition of human NCI-H2126 cell growth in a cell viability assay, IC50=12.3371 μM | ||||
| NCI-H2170 cell | Growth inhibition assay | Inhibition of human NCI-H2170 cell growth in a cell viability assay, IC50=18.1028 μM | ||||
| NCI-H2196 cell | Growth inhibition assay | Inhibition of human NCI-H2196 cell growth in a cell viability assay, IC50=24.8003 μM | ||||
| NCI-H2228 cell | Growth inhibition assay | Inhibition of human NCI-H2228 cell growth in a cell viability assay, IC50=2.28604 μM | ||||
| NCI-H2342 cell | Growth inhibition assay | Inhibition of human NCI-H2342 cell growth in a cell viability assay, IC50=0.93779 μM | ||||
| NCI-H2347 cell | Growth inhibition assay | Inhibition of human NCI-H2347 cell growth in a cell viability assay, IC50=16.1581 μM | ||||
| NCI-H2405 cell | Growth inhibition assay | Inhibition of human NCI-H2405 cell growth in a cell viability assay, IC50=10.4212 μM | ||||
| NCI-H2452 cell | Growth inhibition assay | Inhibition of human NCI-H2452 cell growth in a cell viability assay, IC50=26.6594 μM | ||||
| NCI-H292 cell | Growth inhibition assay | Inhibition of human NCI-H292 cell growth in a cell viability assay, IC50=6.04837 μM | ||||
| NCI-H345 cell | Growth inhibition assay | Inhibition of human NCI-H345 cell growth in a cell viability assay, IC50=16.2706 μM | ||||
| NCI-H358 cell | Growth inhibition assay | Inhibition of human NCI-H358 cell growth in a cell viability assay, IC50=19.7233 μM | ||||
| NCI-H460 cell | Growth inhibition assay | Inhibition of human NCI-H460 cell growth in a cell viability assay, IC50=4.62993 μM | ||||
| NCI-H520 cell | Growth inhibition assay | Inhibition of human NCI-H520 cell growth in a cell viability assay, IC50=1.13303 μM | ||||
| NCI-H522 cell | Growth inhibition assay | Inhibition of human NCI-H522 cell growth in a cell viability assay, IC50=11.0874 μM | ||||
| NCI-H526 cell | Growth inhibition assay | Inhibition of human NCI-H526 cell growth in a cell viability assay, IC50=22.8873μM | ||||
| NCI-H596 | Growth inhibition assay | Inhibition of human NCI-H596 cell growth in a cell viability assay, IC50=17.5708 μM | ||||
| NCI-H630 cell | Growth inhibition assay | Inhibition of human NCI-H630 cell growth in a cell viability assay, IC50=5.51055 μM | ||||
| NCI-H64 cell | Growth inhibition assay | Inhibition of human NCI-H64 cell growth in a cell viability assay, IC50=12.5431 μM | ||||
| NCI-H650 cell | Growth inhibition assay | Inhibition of human NCI-H650 cell growth in a cell viability assay, IC50=7.59877 μM | ||||
| NCI-H661 cell | Growth inhibition assay | Inhibition of human NCI-H661 cell growth in a cell viability assay, IC50=5.42326 μM | ||||
| NCI-H747 cell | Growth inhibition assay | Inhibition of human NCI-H747 cell growth in a cell viability assay, IC50=7.62799 μM | ||||
| NCI-H838 cell | Growth inhibition assay | Inhibition of human NCI-H838 cell growth in a cell viability assay, IC50=31.4118 μM | ||||
| NCI-N87 cell | Growth inhibition assay | Inhibition of human NCI-N87 cell growth in a cell viability assay, IC50=29.3042 μM | ||||
| NH-12 cell | Growth inhibition assay | Inhibition of human NH-12 cell growth in a cell viability assay, IC50=0.60864 μM | ||||
| NKM-1 cell | Growth inhibition assay | Inhibition of human NKM-1 cell growth in a cell viability assay, IC50=2.75923 μM | ||||
| NMC-G1 cell | Growth inhibition assay | Inhibition of human NMC-G1 cell growth in a cell viability assay, IC50=42.6693 μM | ||||
| NOS-1 cell | Growth inhibition assay | Inhibition of human NOS-1 cell growth in a cell viability assay, IC50=1.36953 μM | ||||
| NUGC-3 cell | Growth inhibition assay | Inhibition of human NUGC-3 cell growth in a cell viability assay, IC50=16.5906 μM | ||||
| NY cell | Growth inhibition assay | Inhibition of human NY cell growth in a cell viability assay, IC50=31.1864 μM | ||||
| OAW-42 cell | Growth inhibition assay | Inhibition of human OAW-42 cell growth in a cell viability assay, IC50=7.31565 μM | ||||
| OCI-AML2 cell | Growth inhibition assay | Inhibition of human OCI-AML2 cell growth in a cell viability assay, IC50=33.2531 μM | ||||
| OCUB-M cell | Growth inhibition assay | Inhibition of human OCUB-M cell growth in a cell viability assay, IC50=7.72152 μM | ||||
| OE19 cell | Growth inhibition assay | Inhibition of human OE19 cell growth in a cell viability assay, IC50=7.76684 μM | ||||
| OE33 cell | Growth inhibition assay | Inhibition of human OE33 cell growth in a cell viability assay, IC50=1.88926 μM | ||||
| OMC-1 cell | Growth inhibition assay | Inhibition of human OMC-1 cell growth in a cell viability assay, IC50=9.25098 μM | ||||
| OS-RC-2 cell | Growth inhibition assay | Inhibition of human OS-RC-2 cell growth in a cell viability assay, IC50=8.52307 μM | ||||
| OVCAR-3 cell | Growth inhibition assay | Inhibition of human OVCAR-3 cell growth in a cell viability assay, IC50=1.84415 μM | ||||
| OVCAR-4 cell | Growth inhibition assay | Inhibition of human OVCAR-4 cell growth in a cell viability assay, IC50=30.466 μM | ||||
| P12-ICHIKAWA cell | Growth inhibition assay | Inhibition of human P12-ICHIKAWA cell growth in a cell viability assay, IC50=14.7683 μM | ||||
| P30-OHK cell | Growth inhibition assay | Inhibition of human P30-OHK cell growth in a cell viability assay, IC50=4.536 μM | ||||
| PA-1 cell | Growth inhibition assay | Inhibition of human PA-1 cell growth in a cell viability assay, IC50=9.07615 μM | ||||
| PANC-03-27 cell | Growth inhibition assay | Inhibition of human PANC-03-27 cell growth in a cell viability assay, IC50=8.71082 μM | ||||
| PANC-10-05 cell | Growth inhibition assay | Inhibition of human PANC-10-05 cell growth in a cell viability assay, IC50=14.9959 μM | ||||
| PC-3 cell | Growth inhibition assay | Inhibition of human PC-3 cell growth in a cell viability assay, IC50=10.5135 μM | ||||
| PF-382 cell | Growth inhibition assay | Inhibition of human PF-382 cell growth in a cell viability assay, IC50=11.6828 μM | ||||
| RCC10RGB cell | Growth inhibition assay | Inhibition of human RCC10RGB cell growth in a cell viability assay, IC50=8.6567 μM | ||||
| RD cell | Growth inhibition assay | Inhibition of human RD cell growth in a cell viability assay, IC50=20.6036 μM | ||||
| REH cell | Growth inhibition assay | Inhibition of human REH cell growth in a cell viability assay, IC50=11.4883 μM | ||||
| RERF-LC-MS cell | Growth inhibition assay | Inhibition of human RERF-LC-MS cell growth in a cell viability assay, IC50=26.1347 μM | ||||
| RKO cell | Growth inhibition assay | Inhibition of human RKO cell growth in a cell viability assay, IC50=19.4439 μM | ||||
| RO82-W-1 cell | Growth inhibition assay | Inhibition of human RO82-W-1 cell growth in a cell viability assay, IC50=47.2783 μM | ||||
| RPMI-2650 cell | Growth inhibition assay | Inhibition of human RPMI-2650 cell growth in a cell viability assay, IC50=5.77947 μM | ||||
| RPMI-8402 cell | Growth inhibition assay | Inhibition of human RPMI-8402 cell growth in a cell viability assay, IC50=16.343 μM | ||||
| RS4-11 cell | Growth inhibition assay | Inhibition of human RS4-11 cell growth in a cell viability assay, IC50=6.68645 μM | ||||
| RT-112 cell | Growth inhibition assay | Inhibition of human RT-112 cell growth in a cell viability assay, IC50=6.71952 μM | ||||
| RVH-421 cell | Growth inhibition assay | Inhibition of human RVH-421 cell growth in a cell viability assay, IC50=32.3606 μM | ||||
| RXF393 cell | Growth inhibition assay | Inhibition of human RXF393 cell growth in a cell viability assay, IC50=20.5863 μM | ||||
| SBC-5 cell | Growth inhibition assay | Inhibition of human SBC-5 cell growth in a cell viability assay, IC50=20.8325 μM | ||||
| SCC-25 cell | Growth inhibition assay | Inhibition of human SCC-25 cell growth in a cell viability assay, IC50=15.8179 μM | ||||
| SCC-4 cell | Growth inhibition assay | Inhibition of human SCC-4 cell growth in a cell viability assay, IC50=9.59283 μM | ||||
| SCC-9 cell | Growth inhibition assay | Inhibition of human SCC-9 cell growth in a cell viability assay, IC50=3.08536 μM | ||||
| SF126 cell | Growth inhibition assay | Inhibition of human SF126 cell growth in a cell viability assay, IC50=10.6746 μM | ||||
| SF268 cell | Growth inhibition assay | Inhibition of human SF268 cell growth in a cell viability assay, IC50=15.1387 μM | ||||
| SF295 cell | Growth inhibition assay | Inhibition of human SF295 cell growth in a cell viability assay, IC50=13.6183 μM | ||||
| SIG-M5 cell | Growth inhibition assay | Inhibition of human SIG-M5 cell growth in a cell viability assay, IC50=12.6604 μM | ||||
| SJSA-1 cell | Growth inhibition assay | Inhibition of human SJSA-1 cell growth in a cell viability assay, IC50=36.9072 μM | ||||
| SK-CO-1 cell | Growth inhibition assay | Inhibition of human SK-CO-1 cell growth in a cell viability assay, IC50=10.6394 μM | ||||
| SK-LMS-1 cell | Growth inhibition assay | Inhibition of human SK-LMS-1 cell growth in a cell viability assay, IC50=8.76452 μM | ||||
| SK-LU-1 cell | Growth inhibition assay | Inhibition of human SK-LU-1 cell growth in a cell viability assay, IC50=42.043 μM | ||||
| SK-MEL-24 cell | Growth inhibition assay | Inhibition of human SK-MEL-24 cell growth in a cell viability assay, IC50=13.1675 μM | ||||
| SK-MEL-3 cell | Growth inhibition assay | Inhibition of human SK-MEL-3 cell growth in a cell viability assay, IC50=21.4696 μM | ||||
| SK-MES-1 cell | Growth inhibition assay | Inhibition of human SK-MES-1 cell growth in a cell viability assay, IC50=2.10096 μM | ||||
| human SN12C cell | Growth inhibition assay | Inhibition of human SN12C cell growth in a cell viability assay, IC50=17.1967 μM | ||||
| SNB75 cell | Growth inhibition assay | Inhibition of human SNB75 cell growth in a cell viability assay, IC50=7.8297 μM | ||||
| SNU-387 cell | Growth inhibition assay | Inhibition of human SNU-387 cell growth in a cell viability assay, IC50=4.19544 μM | ||||
| SNU-423 cell | Growth inhibition assay | Inhibition of human SNU-423 cell growth in a cell viability assay, IC50=5.94933 μM | ||||
| SNU-C1 cell | Growth inhibition assay | Inhibition of human SNU-C1 cell growth in a cell viability assay, IC50=2.00948 μM | ||||
| SNU-C2B cell | Growth inhibition assay | Inhibition of human SNU-C2B cell growth in a cell viability assay, IC50=22.2673 μM | ||||
| SR cell | Growth inhibition assay | Inhibition of human SR cell growth in a cell viability assay, IC50=13.0746 μM | ||||
| SU-DHL-1 cell | Growth inhibition assay | Inhibition of human SU-DHL-1 cell growth in a cell viability assay, IC50=6.81543 μM | ||||
| SW1710 cell | Growth inhibition assay | Inhibition of human SW1710 cell growth in a cell viability assay, IC50=1.38892 μM | ||||
| SW780 cell | Growth inhibition assay | Inhibition of human SW780 cell growth in a cell viability assay, IC50=4.89358 μM | ||||
| SW872 cell | Growth inhibition assay | Inhibition of human SW872 cell growth in a cell viability assay, IC50=14.1435 μM | ||||
| SW954 cell | Growth inhibition assay | Inhibition of human SW954 cell growth in a cell viability assay, IC50=5.85783 μM | ||||
| T47D cell | Growth inhibition assay | Inhibition of human T47D cell growth in a cell viability assay, IC50=0.5162 μM | ||||
| 22RV1 cell | Growth inhibition assay | Inhibition of human 22RV1 cell growth in a cell viability assay, IC50=6.61424 μM | ||||
| 23132-87 cell | Growth inhibition assay | Inhibition of human 23132-87 cell growth in a cell viability assay, IC50=0.75938 μM | ||||
| 639-V cell | Growth inhibition assay | Inhibition of human 639-V cell growth in a cell viability assay, IC50=5.8773 μM | ||||
| 697 cell | Growth inhibition assay | Inhibition of human 697 cell growth in a cell viability assay, IC50=7.17746 μM | ||||
| 769-P cell | Growth inhibition assay | Inhibition of human 769-P cell growth in a cell viability assay, IC50=1.10483 μM | ||||
| 8-MG-BA cell | Growth inhibition assay | Inhibition of human 8-MG-BA cell growth in a cell viability assay, IC50=8.63401 μM | ||||
| A4-Fuk cell | Growth inhibition assay | Inhibition of human A4-Fuk cell growth in a cell viability assay, IC50=1.8583 μM | ||||
| A427 cell | Growth inhibition assay | Inhibition of human A427 cell growth in a cell viability assay, IC50=3.07693 μM | ||||
| A498 cell | Growth inhibition assay | Inhibition of human A498 cell growth in a cell viability assay, IC50=1.08134 μM | ||||
| AN3-CA cell | Growth inhibition assay | Inhibition of human AN3-CA cell growth in a cell viability assay, IC50=1.65611 μM | ||||
| AU565 cell | Growth inhibition assay | Inhibition of human AU565 cell growth in a cell viability assay, IC50=1.23917 μM | ||||
| BB30-HNC cell | Growth inhibition assay | Inhibition of human BB30-HNC cell growth in a cell viability assay, IC50=2.86449 μM | ||||
| BxPC-3 cell | Growth inhibition assay | Inhibition of human BxPC-3 cell growth in a cell viability assay, IC50=2.22442 μM | ||||
| C-33-A cell | Growth inhibition assay | Inhibition of human C-33-A cell growth in a cell viability assay, IC50=12.4783 μM | ||||
| C2BBe1 cell | Growth inhibition assay | Inhibition of human C2BBe1 cell growth in a cell viability assay, IC50=13.6679 μM | ||||
| C3A cell | Growth inhibition assay | Inhibition of human C3A cell growth in a cell viability assay, IC50=4.42192 μM | ||||
| CAL-33 cell | Growth inhibition assay | Inhibition of human CAL-33 cell growth in a cell viability assay, IC50=3.43125 μM | ||||
| CAL-51 cell | Growth inhibition assay | Inhibition of human CAL-51 cell growth in a cell viability assay, IC50=7.62841 Μm | ||||
| CAL-54 cell | Growth inhibition assay | Inhibition of human CAL-54 cell growth in a cell viability assay, IC50=4.88625 μM | ||||
| CAL-85-1 cell | Growth inhibition assay | Inhibition of human CAL-85-1 cell growth in a cell viability assay, IC50=5.95309 μM | ||||
| TE-10 cell | Growth inhibition assay | Inhibition of human TE-10 cell growth in a cell viability assay, IC50=9.04225 μM | ||||
| TE-15 cell | Growth inhibition assay | Inhibition of human TE-15 cell growth in a cell viability assay, IC50=6.49393 μM | ||||
| TE-5 cell | Growth inhibition assay | Inhibition of human TE-5 cell growth in a cell viability assay, IC50=4.82452 μM | ||||
| TE-8 cell | Growth inhibition assay | Inhibition of human TE-8 cell growth in a cell viability assay, IC50=24.5243 μM | ||||
| TT cell | Growth inhibition assay | Inhibition of human TT cell growth in a cell viability assay, IC50=5.32724 μM | ||||
| U-118-MG cell | Growth inhibition assay | Inhibition of human U-118-MG cell growth in a cell viability assay, IC50=9.90258 μM | ||||
| U-698-M cell | Growth inhibition assay | Inhibition of human U-698-M cell growth in a cell viability assay, IC50=39.0015 μM | ||||
| U251 cell | Growth inhibition assay | Inhibition of human U251 cell growth in a cell viability assay, IC50=19.204 μM | ||||
| UACC-62 cell | Growth inhibition assay | Inhibition of human UACC-62 cell growth in a cell viability assay, IC50=27.0545 μM | ||||
| UACC-893 cell | Growth inhibition assay | Inhibition of human UACC-893 cell growth in a cell viability assay, IC50=5.29339 μM | ||||
| VMRC-RCZ cell | Growth inhibition assay | Inhibition of human VMRC-RCZ cell growth in a cell viability assay, IC50=3.70955 μM | ||||
| YKG-1 cell | Growth inhibition assay | Inhibition of human YKG-1 cell growth in a cell viability assay, IC50=1.01806 μM | ||||
| CCF-STTG1 cell | Growth inhibition assay | Inhibition of human CCF-STTG1 cell growth in a cell viability assay, IC50=6.81501 μM | ||||
| CCRF-CEM cell | Growth inhibition assay | Inhibition of human CCRF-CEM cell growth in a cell viability assay, IC50=4.93541 μM | ||||
| CESS cell | Growth inhibition assay | Inhibition of human CESS cell growth in a cell viability assay, IC50=2.70089 μM | ||||
| CHP-212 cell | Growth inhibition assay | Inhibition of human CHP-212 cell growth in a cell viability assay, IC50=4.55867 μM | ||||
| COLO-205 cell | Growth inhibition assay | Inhibition of human COLO-205 cell growth in a cell viability assay, IC50=1.99706 μM | ||||
| COR-L279 cell | Growth inhibition assay | Inhibition of human COR-L279 cell growth in a cell viability assay, IC50=1.68064 μM | ||||
| CTB-1 cell | Growth inhibition assay | Inhibition of human CTB-1 cell growth in a cell viability assay, IC50=7.59751 μM | ||||
| Ca-Ski cell | Growth inhibition assay | Inhibition of human Ca-Ski cell growth in a cell viability assay, IC50=5.3411 μM | ||||
| Ca9-22 cell | Growth inhibition assay | Inhibition of human Ca9-22 cell growth in a cell viability assay, IC50=5.97333 μM | ||||
| Calu-1 cell | Growth inhibition assay | Inhibition of human Calu-1 cell growth in a cell viability assay, IC50=7.25613 μM | ||||
| Calu-3 cell | Growth inhibition assay | Inhibition of human Calu-3 cell growth in a cell viability assay, IC50=3.65321 μM | ||||
| DMS-273 cell | Growth inhibition assay | Inhibition of human DMS-273 cell growth in a cell viability assay, IC50=4.15134 μM | ||||
| DOK cell | Growth inhibition assay | Inhibition of human DOK cell growth in a cell viability assay, IC50=4.15134 μM | ||||
| DSH1 cell | Growth inhibition assay | Inhibition of human DSH1 cell growth in a cell viability assay, IC50=0.98722 μM | ||||
| DV-90 cell | Growth inhibition assay | Inhibition of human DV-90 cell growth in a cell viability assay, IC50=6.03122 μM | ||||
| Detroit562 cell | Growth inhibition assay | Inhibition of human Detroit562 cell growth in a cell viability assay, IC50=4.79465 μM | ||||
| DoTc2-4510 cell | Growth inhibition assay | Inhibition of human DoTc2-4510 cell growth in a cell viability assay, IC50=3.43202 μM | ||||
| EB2 cell | Growth inhibition assay | Inhibition of human EB2 cell growth in a cell viability assay, IC50=7.75468 μM | ||||
| ECC12 cell | Growth inhibition assay | Inhibition of human ECC12 cell growth in a cell viability assay, IC50=8.88569 μM | ||||
| EFM-19 cell | Growth inhibition assay | Inhibition of human EFM-19 cell growth in a cell viability assay, IC50=0.56408 μM | ||||
| EFO-21 cell | Growth inhibition assay | Inhibition of human EFO-21 cell growth in a cell viability assay, IC50=4.43468 μM | ||||
| ES7 cell | Growth inhibition assay | Inhibition of human ES7 cell growth in a cell viability assay, IC50=1.34511 μM | ||||
| ETK-1 | Growth inhibition assay | Inhibition of human ETK-1 cell growth in a cell viability assay, IC50=4.57383 μM | ||||
| EW-3 cell | Growth inhibition assay | Inhibition of human EW-3 cell growth in a cell viability assay, IC50=5.64414 μM | ||||
| HCC70 cell | Growth inhibition assay | Inhibition of human HCC70 cell growth in a cell viability assay, IC50=0.75078 μM | ||||
| HGC-27 cell | Growth inhibition assay | Inhibition of human HGC-27 cell growth in a cell viability assay, IC50=0.15033 μM | ||||
| IGROV-1 cell | Growth inhibition assay | Inhibition of human IGROV-1 cell growth in a cell viability assay, IC50=0.72328 Μm | ||||
| MDA-MB-361 cell | Growth inhibition assay | Inhibition of human MDA-MB-361 cell growth in a cell viability assay, IC50=0.48995 μM | ||||
| MDA-MB-415 cell | Growth inhibition assay | Inhibition of human MDA-MB-415 cell growth in a cell viability assay, IC50=1.73449 μM | ||||
| MDA-MB-468 cell | Growth inhibition assay | Inhibition of human MDA-MB-468 cell growth in a cell viability assay, IC50=3.70773 μM | ||||
| ME-180 cell | Growth inhibition assay | Inhibition of human ME-180 cell growth in a cell viability assay, IC50=3.15011 Μm | ||||
| NCI-H526 cells | Growth inhibition assay | Inhibition of Akt-mediated antiapoptotic activity in human NCI-H526 cells assessed as disruption of protein-Apaf-1 complex at 50 uM after 24 hrs by Western blot analysis | ||||
| Clicca per visualizzare più dati sperimentali sulle linee cellulari | ||||||
Informazioni chimiche, conservazione e stabilità
| Peso molecolare | 551.64 | Formula | C34H29N7O |
Conservazione (Dalla data di ricezione) | |
|---|---|---|---|---|---|
| N. CAS | 612847-09-3 | Scarica SDF | Conservazione delle soluzioni stock |
|
|
| Sinonimi | Akt Inhibitor VIII | Smiles | C1CN(CCC1N2C3=CC=CC=C3NC2=O)CC4=CC=C(C=C4)C5=C(N=C6C=C7C(=NC=N7)C=C6N5)C8=CC=CC=C8 | ||
Solubilità
|
In vitro |
DMSO
: 22 mg/mL
(39.88 mM)
Water : Insoluble Ethanol : Insoluble |
Calcolatore di Molarità
Calcolatore di Diluizione
Calcolatore del Peso Molecolare
|
In vivo |
|||||
Calcolatore di formulazione in vivo (Soluzione chiara)
Passo 1: Inserire le informazioni di seguito (Consigliato: Un animale aggiuntivo per tenere conto della perdita durante lesperimento)
mg/kg
g
μL
Passo 2: Inserire la formulazione in vivo (Questo è solo il calcolatore, non la formulazione. Contattateci prima se non cè una formulazione in vivo nella sezione Solubilità.)
% DMSO
%
% Tween 80
% ddH2O
%DMSO
%
Risultati del calcolo:
Concentrazione di lavoro: mg/ml;
Metodo per preparare il liquido master di DMSO: mg farmaco predissolto in μL DMSO ( Concentrazione del liquido master mg/mL, Vi preghiamo di contattarci prima se la concentrazione supera la solubilità del DMSO del lotto del farmaco. )
Metodo per preparare la formulazione in vivo: Prendere μL DMSO liquido master, quindi aggiungereμL PEG300, mescolare e chiarire, quindi aggiungereμL Tween 80, mescolare e chiarire, quindi aggiungere μL ddH2O, mescolare e chiarire.
Metodo per preparare la formulazione in vivo: Prendere μL DMSO liquido master, quindi aggiungere μL Olio di mais, mescolare e chiarire.
Nota: 1. Si prega di assicurarsi che il liquido sia limpido prima di aggiungere il solvente successivo.
2. Assicurarsi di aggiungere il/i solvente/i in ordine. È necessario assicurarsi che la soluzione ottenuta, nellaggiunta precedente, sia una soluzione limpida prima di procedere allaggiunta del solvente successivo. Metodi fisici come il vortex, gli ultrasuoni o il bagno dacqua calda possono essere utilizzati per facilitare la dissoluzione.
Meccanismo dazione
| Targets/IC50/Ki |
Akt1
(Cell-free assay) 58 nM
Akt2
(Cell-free assay) 210 nM
Akt3
(Cell-free assay) 2119 nM
|
|---|---|
| In vitro |
In un saggio IPKA (C33A) basato su cellule, Akti-1/2 inibisce Akt1 e Akt2 con IC50 di 305 nM e 2086 nM, rispettivamente. Nelle cellule HT29, MCF7 e A2780, questo composto induce l'apoptosi cellulare aumentando drasticamente l'attività della caspasi-3. Nelle cellule epatiche, questa sostanza chimica blocca la regolazione dell'espressione di PEPCK, G6Pase e l'attività di FOXO1. Potenzia anche fortemente l'aggregazione piastrinica mediata da PAR-1 bloccando la PKB. Nelle cellule HCC827, NCI-H522, NCI-1651 e PC-9, questo inibitore inibisce la crescita cellulare con IC50 di 4,7 μM, 7,25 μM e 9,5 μM; in combinazione, provoca una maggiore inibizione della crescita cellulare e dell'apoptosi. |
| Saggio chinasico |
Screen di chinasi
|
|
In breve, tutti i saggi (25,5 μl a 21 °C per 30 min) vengono eseguiti utilizzando una workstation di automazione di laboratorio Biomek 2000 in un formato a 96 pozzetti. Le reazioni contengono 5
–20 mU di chinasi purificata insieme a un peptide o proteina substrato e vengono avviate con l'aggiunta di 10 mM di MgAcetato e 5, 20 o 50 μM di ATP ([γ-33P]-ATP, 800 cpm/pmol).
|
|
| In vivo |
Nei topi, Akti-1/2 (50 mg/kg, i.p.) inibisce la fosforilazione basale e stimolata da IGF di Akt1 e Akt2 nel polmone. |
Riferimenti |
|
Applicazioni
| Metodi | Biomarcatori | Immagini | PMID |
|---|---|---|---|
| Western blot | p-AKT / AKT / p-Foxo3a / p-GSK3β / p-p70S6K / p-S6 / p-4EBP1 / Cyclin D1 / Cyclin D2 / Cyclin D3 / p-Rb / p27 |
|
18725974 |
| Immunofluorescence | MCM2 / H3K9me2 / CD63 |
|
26637368 |
Supporto tecnico
Istruzioni per la manipolazione
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