Name: Isoliquiritigenin
Brand: Selleck Chemicals
SKU: S2404
Rating: 5 (10 reviews)

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Controllo Qualità

Lotto: Purezza: 99.97%

99.97

Target correlati	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Altro Aldose Reductase Inibitori	Epalrestat Alrestatin Calceolarioside B Obtusifolin Poliumoside Soyasaponin Bb

Coltura cellulare, trattamento e concentrazione di lavoro

Linee cellulari	Tipo di saggio	Concentrazione	Tempo di incubazione	Descrizione dellattività
KB cells	Growth inhibition assay			Compound concentration required to reduce the exponential growth of KB cells by 50%, CC50=12 μM
MT-4 cells	Growth inhibition assay			Compound concentration required to reduce the exponential growth of MT-4 cells by 50%, CC50=7.4 μM
Hepa lclc7 cells	Cytotoxicity assay			Cytotoxicity against mouse Hepa lclc7 cells, IC50=39 μM
J774.1 cells	Function assay		24 h	Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs by Griess reagent assay, IC50=29.3 μM
colon 26-L5 cells	Cytotoxicity assay		72 h	Cytotoxicity against mouse colon 26-L5 cells after 72 hrs by MTT assay, IC50=21.8 μM
MCF7 cells	Cytotoxicity assay	25-150 μM	72 h	Cytotoxicity against human MCF7 cells at 25 to 150 uM after 72 hrs by MTT assay
CHO cells	Cytotoxicity assay			Cytotoxicity against CHO cells by MTT assay, IC50=45.9 μM
human T47D cells	Function assay		96 h	Estrogenic activity in human T47D cells assessed as drug level causing stimulation of cell proliferation equivalent to 10 pM estradiol after 96 hrs by alamar blue assay
MCF7 cells	Function assay		96 h	Estrogenic activity in human MCF7 cells assessed as drug level causing stimulation of cell proliferation equivalent to 10 pM estradiol after 96 hrs by alamar blue assay
MCF7 cells	Function assay			Estrogenic activity in luciferase transfected human MCF7 cells assessed as drug level causing stimulation of cell proliferation equivalent to 10 pM estradiol by luciferase reporter gene assay
mouse NIH3T3 cells	Function assay		8 h	Inhibition of cobalt chloride-induced HIF-1 activation expressed in mouse NIH3T3 cells after 8 hrs by luciferase reporter gene assay, IC50=4.8 μM
OE21 cells	Cytotoxicity assay		72 h	Cytotoxicity against human OE21 cells after 72 hrs by MTT assay, IC50=48 μM
U937 cells	Function assay	5 ug/ml	24 h	Antiinflammatory activity in human U937 cells assessed as inhibition of LPS-induced CCL5 secretion at 5 ug/ml after 24 hrs by ELISA relative to control
dog MDCK cells	Function assay	20 ug/mL	1 h	Antiviral activity against Influenza virus A/Puerto Rico/8/34 H1N1 infected in dog MDCK cells assessed as inhibition of viral replication at 20 ug/mL incubated at 1 hr post-infection measured after 24 hrs by hemagglutininating unit assay relative to control
dog MDCK cells	Function assay		8 h	Inhibition of PKC/p38MAPK-mediated nuclear-cytoplasmic viral ribonucleoprotein export in dog MDCK cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 8 hrs post-infection by inmmunofluorescence
NCI-H292 cells	Function assay			Inhibition of PKC-mediated PKD phosphorylation in human NCI-H292 cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 6 hrs post-infection by immunoblotting analysis
NCI-H292 cells	Function assay			Inhibition of PKC-mediated p38MAPK phosphorylation in human NCI-H292 cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 6 hrs post-infection by immunoblotting analysis
NCI-H292 cells	Function assay			Inhibition of PKC-mediated JNK phosphorylation in human NCI-H292 cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 6 hrs post-infection by immunoblotting analysis
dog MDCK cells	Function assay			Restoration of GSH level in dog MDCK cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 24 hrs post-infection by DTNB-based colometric assay
RBL-1 cells	Function assay			In vitro inhibition against 5-lipoxygenase in RBL-1 cells was determined, IC50=35 μM
PC3 cells	Cytotoxicity assay			Cytotoxicity against human PC3 cells, IC50=46.4 μM
HUVEC	Cytotoxicity assay		48 h	Toxicity against HUVEC incubated for 48 hrs by MTT assay, IC50=41.17 μM
K562 cells	Cytotoxicity assay		48 h	Antitumor activity against human K562 cells incubated for 48 hrs by MTT assay, IC50=29.27 μM
Hepa-1c1c7 cells	Function assay			Induction of quinone reductase activity in mouse Hepa-1c1c7 cells assessed as drug level required to double enzyme activity
Hepa lclc7 cells	Function assay			Induction of mouse quinone reductase in mouse Hepa lclc7 cells assessed as concentration required to double enzyme activity
U937 cells	Function assay	5 ug/ml		Inhibition of LPS-induced AP-1 activation in human U937 cells at 5 ug/ml after 1 hr relative to control
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Informazioni chimiche, conservazione e stabilità

Peso molecolare	256.25	Formula	C₁₅H₁₂O₄	Conservazione (Dalla data di ricezione)	3 anni-20°Cpolvere
N. CAS	961-29-5	Scarica SDF		Conservazione delle soluzioni stock	1 anno-80°Cin solvente 1 mese-20°Cin solvente
Sinonimi	N/A			Smiles	C1=CC(=CC=C1C=CC(=O)C2=C(C=C(C=C2)O)O)O

Solubilità

In vitro
Lotto:

Ethanol : 26 mg/mL

DMSO : 12 mg/mL (46.82 mM)
(Il DMSO contaminato da umidità può ridurre la solubilità. Utilizzare DMSO fresco e anidro.)

Water : Insoluble

Calcolatore di Molarità

Massa	Concentrazione	Volume	Peso molecolare
=	×	×

Calcolatore di Diluizione Calcolatore del Peso Molecolare

In vivo Lotto:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validato dai laboratori Selleck. Se necessiti di modifiche a questa formulazione, contatta il nostro team di vendita per test personalizzati.	0.250mg/ml (0.98mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validato dai laboratori Selleck. Se necessiti di modifiche a questa formulazione, contatta il nostro team di vendita per test personalizzati.	2.500mg/ml (9.76mM)	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

Calcolatore di formulazione in vivo (Soluzione chiara)

Passo 1: Inserire le informazioni di seguito (Consigliato: Un animale aggiuntivo per tenere conto della perdita durante lesperimento)

Dosaggio: mg/kg Peso medio degli animali: g Volume di dosaggio per animale:μL Numero di animali:

Passo 2: Inserire la formulazione in vivo (Questo è solo il calcolatore, non la formulazione. Contattateci prima se non cè una formulazione in vivo nella sezione Solubilità.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Risultati del calcolo:

Concentrazione di lavoro: mg/ml;

Metodo per preparare il liquido master di DMSO: mg farmaco predissolto in μL DMSO ( Concentrazione del liquido master mg/mL, Vi preghiamo di contattarci prima se la concentrazione supera la solubilità del DMSO del lotto del farmaco. )

Metodo per preparare la formulazione in vivo: Prendere μL DMSO liquido master, quindi aggiungereμL PEG300, mescolare e chiarire, quindi aggiungereμL Tween 80, mescolare e chiarire, quindi aggiungere μL ddH₂O, mescolare e chiarire.

Metodo per preparare la formulazione in vivo: Prendere μL DMSO liquido master, quindi aggiungere μL Olio di mais, mescolare e chiarire.

Nota: 1. Si prega di assicurarsi che il liquido sia limpido prima di aggiungere il solvente successivo.
2. Assicurarsi di aggiungere il/i solvente/i in ordine. È necessario assicurarsi che la soluzione ottenuta, nellaggiunta precedente, sia una soluzione limpida prima di procedere allaggiunta del solvente successivo. Metodi fisici come il vortex, gli ultrasuoni o il bagno dacqua calda possono essere utilizzati per facilitare la dissoluzione.

Meccanismo dazione

Targets/IC₅₀/Ki	Aldose reductase (Cell-free assay) 320 nM
In vitro	Isoliquiritigenin può essere efficace nella prevenzione delle complicanze diabetiche attraverso l'inibizione dell'aldoso reduttasi del cristallino di ratto con un IC50 di 320 nM e l'accumulo di sorbitolo nei globuli rossi umani con un IC50 di 2,0 μM. Questo composto può anche servire come agente schiarente per la pelle inibendo le attività tirosinasiche mono- e difenolase con un IC50 di 8,1 μM. Induce l'arresto del ciclo cellulare e l'inibizione della crescita cellulare nelle linee cellulari di cancro alla prostata DU145 e LNCaP. Questa sostanza chimica induce l'apoptosi delle cellule MGC-803 del cancro gastrico umano aumentando la concentrazione intracellulare di calcio libero e diminuendo il potenziale transmembrana mitocondriale (Deltapsi(m)).
In vivo	DL50: Topi >6g/kg (i.g.)
Riferimenti	[1] https://pubmed.ncbi.nlm.nih.gov/2118267/ [2] https://pubmed.ncbi.nlm.nih.gov/12590456/ [3] https://pubmed.ncbi.nlm.nih.gov/12706007/ [4] https://pubmed.ncbi.nlm.nih.gov/11731922/ [5] https://pubmed.ncbi.nlm.nih.gov/19067384/

Supporto tecnico

Istruzioni per la manipolazione

Tel: +1-832-582-8158 Ext:3

Per qualsiasi altra domanda, si prega di lasciare un messaggio.

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Isoliquiritigenin Aldose Reductase inibitore

Struttura chimica

Peso molecolare: 256.25