Melatonin

N. catalogoS1204 Lotto:S120406

Stampa

Dati tecnici

Formula

C13H16N2O2
Peso molecolare 232.28 Numero CAS 73-31-4
Solubilità (25°C)* In vitro DMSO 46 mg/mL (198.03 mM)
Ethanol 46 mg/mL (198.03 mM)
Water Insoluble
In vivo (Aggiungere i solventi al prodotto singolarmente e in ordine.)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%absolute ethyl alcohol 95%Corn oil

Convalidato dai laboratori Selleck. Se sono necessarie modifiche a questa formulazione, contattare il nostro team di vendita per test personalizzati.

 2.300mg/ml (9.90mM) Taking the 1 mL working solution as an example, add 50 μL of 46 mg/ml clear absolute ethyl alcohol stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml significa leggermente solubile o insolubile.
* Si prega di notare che Selleck testa la solubilità di tutti i composti internamente e la solubilità effettiva può differire leggermente dai valori pubblicati. Ciò è normale ed è dovuto a leggere variazioni da lotto a lotto.
* Spedizione a temperatura ambiente (I test di stabilità mostrano che questo prodotto può essere spedito senza alcuna misura di raffreddamento.)

Preparazione delle soluzioni stock

Attività biologica

Descrizione Melatonin è un agonista del MT receptor, usato come integratore alimentare. Questo composto è un inibitore selettivo di ATF-6 e downregola COX-2. Migliora la mitophagy e regola l'omeostasi dell'apoptosis e dell'autophagy.
Target
melatonin receptor
In vitro

Melatonin interacts with the highly toxic hydroxyl radical with a rate constant equivalent to that of other highly efficient hydroxyl radical scavengers. It reportedly neutralizes hydrogen peroxide, singlet oxygen, peroxynitrite anion, nitric oxide and hypochlorous acid.

This compound is believed to scavenge the highly toxic hydroxyl radical, the peroxynitrite anion, and possibly the peroxyl radical. It reportedly scavenges the superoxide anion radical and it quenches singlet oxygen. The substance stimulates mRNA levels for superoxide dismutase and the activities of glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase (all of which are antioxidative enzymes), thereby increasing its antioxidative capacity.

This chemical in cell-free systems has been shown to directly scavenge H2O2, singlet oxygen (1O2) and nitric oxide (NO*), with little or no ability to scavenge the superoxide anion radical (O2*-) in vitro. It also directly detoxifies the peroxynitrite anion (ONOO-) and/or peroxynitrous acid (ONOOH), or the activated form of this molecule, ONOOH*. Melatonin acts as a direct free radical scavenger with the ability to detoxify both reactive oxygen and reactive nitrogen species.

It inhibits cAMP accumulation in most of the cells examined, but the indole effects on other messengers have been often observed only in one type of the cells or tissue, until now. This compound also regulates the transcription factors, namely, phosphorylation of cAMP-responsive element binding protein and expression of c-Fos.

In vivo Melatonin ameliorates necrotizing enterocolitis by preventing Th17/Treg imbalance through activation of the AMPK/SIRT1 pathway

Protocollo (da riferimento)

Saggio cellulare:

[5]
  • Linee cellulari

    HepG2 cells

  • Concentrazioni

    1.2 mM

  • Tempo di incubazione

    24 h

  • Metodo

    Cells were treated with various concentrations of this compound for 24 h.
Studio sugli animali:

[6]
  • Modelli animali

    C57BL/6 mice

  • Dosaggi

    10 mg/kg

  • Somministrazione

    i.p.

Riferimenti

  • https://pubmed.ncbi.nlm.nih.gov/11060493/
  • https://pubmed.ncbi.nlm.nih.gov/9770244/
  • https://pubmed.ncbi.nlm.nih.gov/11898866/
  • https://pubmed.ncbi.nlm.nih.gov/9674691/
  • https://pubmed.ncbi.nlm.nih.gov/36225557/
  • https://pubmed.ncbi.nlm.nih.gov/32685016/

Convalida del prodotto da parte del cliente

<p>TUNEL staining of treated adipocytes and flow cytometry analysis of positive TUNEL cells (n=3).</p>

, , J Pineal Res. 2017, 62(4), doi: 10.1111/jpi.12383

C: EDU staining of adipocytes treated with MT for 24 h (n=3). D: Flow Cytometry analysis of cell cycle in adipocytes treated with MT for 24 h (n=3).

Dati da [ , , J Pineal Res, 2016, doi: 10.1111/jpi.12383. ]

H2O2 activates JNK through the RAC1/MAP2K7 pathway, which is inhibited by melatonin treatment. (A) GCs grown in medium containing 10 μM melatonin for 24 h were rinsed using PBS, and then incubated with 0.2 mM H2O2 for 2 h. For the inhibition of RAC1, the RAC1 specific antagonist NSC 23766 (10 μM) was added 1 h prior to H2O2 exposure. The cell lysates were collected for GST-PAK1-PBD pulldown assay of RAC1 activation (GTP-bound RAC1 levels) and immunoblotting analysis of total RAC1. (B) Western blot analysis of phosphorylated MAP2K7 (p-MAP2K7) and total MAP2K7 in GCs with the indicated treatments as described above. TUBA1A served as the control for loading. (C) The detection of JNK activity in GCs subjected to the treatments as mentioned earlier. Data represent mean ± s.e.; n = 3. **Represents P < 0.01 vs control group. ##Represents P < 0.01 vs H2O2 group. N, not significant, P > 0.05. (D) GCs transfected with scrambled control siRNA (SC siRNA) or Map2k7 siRNA were cultured with 10 μM melatonin for 24 h, washed in PBS, and then grown in medium containing 0.2 mM H2O2 for 2 h. The expression of phosphorylated MAP2K7 (p-MAP2K7) and total MAP2K7 was determined by Western blotting. TUBA1A served as the control for loading. (E) GCs transfected with scrambled control siRNA (SC siRNA) or Map2k7 siRNA for 48 h were cultured with or without 0.2 mM H2O2 for 2 h. Cell lysates were collected for Western blot analysis of RAC1 activation (GTP-RAC1 levels) by GST-PAK1-PBD pulldown and of total RAC1 levels. (F) GCs transfected with scrambled control siRNA (SC siRNA) or Map2k7 siRNA for 24 h were cultured for another 24 h in the presence or absence of 10 μM melatonin before 2 h of H2O2 (0.2 mM) incubation. Cells were then processed for JNK determination. Data represent mean ± s.e.; n = 3. **Represents P < 0.01 vs control group. ##Represents P < 0.01 vs H2O2 group. N, not significant, P > 0.05.

Dati da [ , , Reproduction, 2018, 155(3):307-319 ]

(a-c) Immunohistochemical detection of Nrf2, HO-1 and NQO1 in lumber enlargement of mice from different groups at the peak stage of EAE or on day 28 post immunization.

Dati da [ , , J Mol Neurosci, 2018, 64(2):233-241 ]

Selleck's Melatonin È stato citato da 39 Pubblicazioni

Zearalenone Exposure Damages Skeletal Muscle Through Oxidative Stress and Is Alleviated by Glutathione, Nicotinamide Mononucleotide, and Melatonin [ Antioxidants (Basel), 2025, 14(5)528] PubMed: 40427410
Melatonin suppresses PD-L1 expression and exerts antitumor activity in hepatocellular carcinoma [ Sci Rep, 2025, 15(1):8451] PubMed: 40069331
Melatonin ameliorates ischemic brain injury in experimental stroke by regulation of miR-221 and ATG7 axis [ Biochem Biophys Res Commun, 2025, 760:151706] PubMed: 40157291
Melatonin alleviates aging-related heart failure through melatonin receptor 1A/B knockout in mice [ Heliyon, 2024, 10(18):e38098] PubMed: 39347399
Ciprofloxacin is a novel anti-ferroptotic antibiotic [ Heliyon, 2024, 10(11):e32571] PubMed: 38961954
Experimental study on small molecule combinations inducing reprogramming of rat fibroblasts into functional neurons [ Zhejiang Da Xue Xue Bao Yi Xue Ban, 2024, 53(4):498-508] PubMed: 39183062
Melatonin ameliorates glyphosate- and hard water-induced renal tubular epithelial cell senescence via PINK1-Parkin-dependent mitophagy [ Ecotoxicol Environ Saf, 2023, 255:114719] PubMed: 37032573
Melatonin attenuates chronic intermittent hypoxia-induced intestinal barrier dysfunction in mice [ Microbiol Res, 2023, 276:127480] PubMed: 37659335
Notum enhances gastric cancer stem-like cell properties through upregulation of Sox2 by PI3K/AKT signaling pathway [ Cell Oncol (Dordr), 2023, 10.1007/s13402-023-00875-w] PubMed: 37749430
Synergistic Renoprotective Effect of Melatonin and Zileuton by Inhibition of Ferroptosis via the AKT/mTOR/NRF2 Signaling in Kidney Injury and Fibrosis [ Biomol Ther (Seoul), 2023, 10.4062/biomolther.2023.062] PubMed: 37183002

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