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Cyclophosphamide Monohydrate Alkylating Agent

N. Cat.: S2057

Cyclophosphamide Monohydrate is a nitrogen mustard alkylating agent, it attaches the alkyl group to the guanine base of DNA, shown to crosslink DNA, causing strand breakage and inducing mutations.Cyclophosphamide (NSC-26271) Monohydrate can be used to induce animal models of Anemia.
Cyclophosphamide Monohydrate Alkylating Agent inhibitor Chemical Structure

Struttura chimica

Peso molecolare: 279.1

Vai a

Controllo Qualità (Quality Control)

Lotto: Purezza: 99.20%
99.20

Coltura cellulare, trattamento e concentrazione di lavoro
(Cell Culture, Treatment & Working Concentration)

Linee cellulari Tipo di saggio Concentrazione Tempo di incubazione Formulazione Descrizione dell'attività PMID
HL60 cells Cytotoxicity assay Cytotoxicity against human HL60 cells by MTT assay, IC50=8.79 μM 20850303
BALB/c 3T3 cells Cytotoxicity assay In vitro cytotoxicity was evaluated in mouse embryo BALB/c 3T3 cells, IC50=37.6 μM 9873412
BALB/c 3T3 Cytotoxicity assay In vitro cytotoxicity against BALB/c 3T3 cells, IC50 = 37.6 μM. 7877150
T-cells Immunosuppressive assay 100 mg/kg 8 days Immunosuppressive activity against MAV-1 infected BALB/c mouse assessed as T cells suppression at 100 mg/kg, ip treated 8 days before infection for 4 weeks measured 14 days post infection by flow cytometry 18268085
B-cells Immunosuppressive assay 100 mg/kg 8 days Immunosuppressive activity against MAV-1 infected BALB/c mouse assessed as B cells suppression at 100 mg/kg, ip treated 8 days before infection for 4 weeks measured 14 days post infection by flow cytometry 18268085
U87 MG Antitumor assay 80 mg/kg Antitumor activity in human U87 MG cells xenografted mouse at 80 mg/kg, iv administered in Q2D x 5 schedule 18450456
MX1 Antitumor assay 120 mg/kg up to 3 weeks Antitumor activity against human MX1 cells xenografted in nude mouse adjuvant model assessed as increase in mouse survival time at 120 mg/kg, po BID measured up to 3 weeks 20726512
U87MG Anticancer assay 80 mg/kg 6 days Anticancer activity against human U87MG cells xenografted in athymic mouse assessed as tumor suppression at 80 mg/kg, iv Q2D for 6 days 21106377
NCI-H522 Antitumor assay 50 mg/kg 28 days Antitumor activity against human NCI-H522 cells xenografted in Balb/c nude mouse assessed as tumor growth inhibition at 50 mg/kg, ig administered once daily for 28 days relative to control 28092860
U2932 Antitumor assay 50 mg/kg 5 consecutive days Antitumor activity against human U2932 cells xenografted in SCID mouse assessed as reduction in tumor burden at 50 mg/kg, ip administered for 5 consecutive days measured up to day 40 post cell injection 28605593
MDA-MB-231 Cytotoxicity assay 48 hr Cytotoxicity against Homo sapiens (human) MDA-MB-231 cells after 48 hr by MTT assay, IC50 = 0.09 μM. ChEMBL
K562 Cytotoxicity assay 48 hr Cytotoxicity against Homo sapiens (human) K562 cells after 48 hr by MTT assay, IC50 = 0.15 μM. ChEMBL
K562 Antiproliferative assay 48 hr Antiproliferative activity against Homo sapiens (human) K562 cells after 48 hr by MTT assay, IC50 = 0.153 μM. ChEMBL
MCF7 Cytotoxicity assay 48 hr Cytotoxicity against Homo sapiens (human) MCF7 cells after 48 hr by SRB assay, IC50 = 10 mM. 19372630
HepG2 Cytotoxicity assay 48 hr Cytotoxicity against Homo sapiens (human) HepG2 cells after 48 hr by MTT assay, IC50 = 0.24 μM. ChEMBL
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Informazioni chimiche, conservazione e stabilità (Chemical Information, Storage & Stability)

Peso molecolare 279.1 Formula

C7H15Cl2N2O2P.H2O

 Conservazione (Dalla data di ricezione)
N. CAS 6055-19-2 Scarica SDF Conservazione delle soluzioni stock

Sinonimi NSC-26271 Monohydrate Smiles C1CNP(=O)(OC1)N(CCCl)CCCl.O

Solubilità (Solubility)

In vitro
Lotto:

DMSO : 55 mg/mL (197.06 mM)
(Il DMSO contaminato da umidità può ridurre la solubilità. Utilizzare DMSO fresco e anidro.)

Water : 55 mg/mL

Ethanol : 55 mg/mL

Calcolatore di Molarità

Massa Concentrazione Volume Peso molecolare
Calcolatore di Diluizione Calcolatore del Peso Molecolare

In vivo
Lotto:

Calcolatore di formulazione in vivo (Soluzione chiara)

Passo 1: Inserire le informazioni di seguito (Consigliato: Un animale aggiuntivo per tenere conto della perdita durante l'esperimento)

mg/kg g μL

Passo 2: Inserire la formulazione in vivo (Questo è solo il calcolatore, non la formulazione. Contattateci prima se non c'è una formulazione in vivo nella sezione Solubilità.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Risultati del calcolo:

Concentrazione di lavoro: mg/ml;

Metodo per preparare il liquido master di DMSO: mg farmaco predissolto in μL DMSO ( Concentrazione del liquido master mg/mL, Vi preghiamo di contattarci prima se la concentrazione supera la solubilità del DMSO del lotto del farmaco. )

Metodo per preparare la formulazione in vivo: Prendere μL DMSO liquido master, quindi aggiungereμL PEG300, mescolare e chiarire, quindi aggiungereμL Tween 80, mescolare e chiarire, quindi aggiungere μL ddH2O, mescolare e chiarire.

Metodo per preparare la formulazione in vivo: Prendere μL DMSO liquido master, quindi aggiungere μL Olio di mais, mescolare e chiarire.

Nota: 1. Si prega di assicurarsi che il liquido sia limpido prima di aggiungere il solvente successivo.
2. Assicurarsi di aggiungere il/i solvente/i in ordine. È necessario assicurarsi che la soluzione ottenuta, nell'aggiunta precedente, sia una soluzione limpida prima di procedere all'aggiunta del solvente successivo. Metodi fisici come il vortex, gli ultrasuoni o il bagno d'acqua calda possono essere utilizzati per facilitare la dissoluzione.

Meccanismo d'azione (Mechanism of Action)

In vitro

Cyclophosphamide (CY) is a chemotherapeutic agent with a dose-dependent, bimodal effect on the immune system. Cyclophosphamide treatment enhances apoptosis and decreases homeostatic proliferation of regulatory T cells. Cyclophosphamide downregulates the expression of GITR and FoxP3, which are involved in the suppressive activity of T(REGs).

Cyclophosphamide increases CYP3A4, CYP2C8, and CYP2C9 protein levels in primary human hepatocyte cultures, which thereby enhances their own rates of 4-hydroxylation in the cultured hepatocytes.

Cyclophosphamide has produced mutations in base-pair substituting strains of Salmonella tryphimurium in the presence of metabolic activation, but it has been shown to be negative in the E. coli chromotest. Cyclophosphamide has been shown to produce gene mutations, chromosome aberrations, micronuclei and sister chromatid exchanges in a variety of cultured cells in the presence of metabolic activation as well as sister chromatid exchanges without metabolic activation.

In vivo

Cyclophosphamide has also produced chromosome damage and micronuclei in rats, mice and Chinese hamsters, and gene mutations in the mouse spot test and in the transgenic lacZ construct of Muta Mouse.

Cyclophosphamide, when given in a defined sequence with a GM-CSF-secreting, neu-expressing whole-cell vaccine, enhances the vaccine's potential to delay tumor growth in neu transgenic mice. Cyclophosphamide mediates its effects by enhancing the efficacy of the vaccine rather than via a direct cytolytic effect on cancer cells.

Riferimenti
  • [4] https://pubmed.ncbi.nlm.nih.gov/11325840/
  • [5] https://pubmed.ncbi.nlm.nih.gov/35409194/
  • [6] https://pubmed.ncbi.nlm.nih.gov/34385713/
  • [7] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7123643/
  • [8] https://pubmed.ncbi.nlm.nih.gov/21530682/

Applicazioni (Applications)

Metodi Biomarcatori Immagini PMID
Western blot PTEN / pAkt / Caspase 3 / GAPDH Caspase 3 / Cleaved-Caspase 3 / Cleaved-PARP / Tubulin Nuclear AIF / Cytosolic AIF / TBP / GAPDH LC3B I / LC3B II / GAPDH
S2057-WB-1
23874108
Growth inhibition assay Cell Viability Tumor Volume Tumor Volume
S2057-Growth-inhibition-assay-1
31429028
IHC tumor cell invasion TUNEL / Caspase 3 PTEN / pAkt / PCNA ovary of mice Caspase-3
S2057-IHC-1
23874108
Immunofluorescence pAKT / pERK Ki-67 / UPK3 / KRT5 / pERK Ki-67 / KRT14 / KRT5 autophagy levels
S2057-IF-1
31654636

Informazioni sullo studio clinico (Clinical Trial Information)

(dati da https://clinicaltrials.gov, aggiornato il 2024-05-22)

Numero NCT Reclutamento Condizioni Sponsor/Collaboratori Data di inizio Fasi
NCT06186700 Active not recruiting
Breast Cancer Female
Mansoura University
 December 25 2023 Phase 2
NCT06085742 Recruiting
Breast Cancer
University of Illinois at Chicago
 November 22 2023 Phase 2
NCT05800041 Not yet recruiting
Gout Tophus
RenJi Hospital|Westlake Therapeutics
 April 10 2023 Early Phase 1

Domande Frequenti (Frequently Asked Questions)

Domanda 1:
Why does it show no activity in vitro assays?

Risposta:
The activity of this compound in vitro is controversial and has not been fully determined. It is a prodrug and may need Cytochrome P450 to convert it to the active form: 4-hydroxy cyclophosphamide. It is widely used in vivo, if you are going to use it in vitro, you may need to supplement Cytochrome P450 exogenously.