Name: Puromycin Dihydrochloride
Brand: Selleck Chemicals
SKU: S7417
Rating: 5 (210 reviews)

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Batch: Zuiverheid: 99.89%

99.89

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Gerelateerde doelwitten	Integrase Bacterial Antibiotics Anti-infection Fungal Antiviral COVID-19 Parasite Reverse Transcriptase HIV
Overige Antineoplastic and Immunosuppressive Antibiotics Inhibitoren	Staurosporine (STS) Cyclosporin A Oligomycin A (MCH 32) Nigericin sodium salt Geldanamycin (NSC 122750) Honokiol Streptozotocin (STZ) Sodium Monensin (NSC 343257) Cephalomannine Pirarubicin

Celkweek, behandeling & werkconcentratie

Cellijnen	Assaytype	Incubatietijd	Activiteitsbeschrijving	PMID
K562	Growth inhibition assay	72 h	Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay, IC50=26.83 μM.	18313307
HepG2	Growth inhibition assay	72 h	Growth inhibition of human HepG2 cells after 72 hrs by MTT assay, IC50=0.23 μM.	18313307
K562	Growth inhibition assay	72 h	Growth inhibition of human K562 cells after 72 hrs by SRB assay, IC50=0.22 μM.	18313307
HepG2	Growth inhibition assay	72 h	Growth inhibition of human HepG2 cells after 72 hrs by SRB assay, IC50=0.21 μM.	18512984
Huh7	Cytotoxicity assay		NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7), CC50=6.63 μM.	18579783
HEK239	Cytotoxicity assay	72 h	Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay, IC50=0.42 μM.	20558060
J774A1	Cytotoxicity assay		Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay, IC50=10 μM.	22934636
KB3-1	Growth inhibition assay	72 hrs	Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay, IC50 = 0.21 μM.	18313307
HepG2/Dox	Growth inhibition assay	72 hrs	Growth inhibition of multidrug resistant human HepG2/Dox cells after 72 hrs by SRB assay, IC50 = 20.2 μM.	18512984
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis, IC50 = 0.361 μM.	26651537
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay, IC50 = 0.3505 μM.	27060763
A549	Cytotoxicity assay		Cytotoxicity against human A549 cells, IC50 = 0.6 μM.	27089214
PC3	Cytotoxicity assay		Cytotoxicity against human PC3 cells, IC50 = 0.7 μM.	27089214
HEK293	Cytotoxicity assay		Cytotoxicity against HEK293 cells by alamar blue assay, IC50 = 0.00035 μM.	27212070
HEK293	Growth inhibition assay	72 hrs	Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay, IC50 = 0.36 μM.	28001067
HEK293	Growth inhibition assay		Growth inhibition of HEK293 cells, IC50 = 0.399 μM.	28400231
SU-DHL10	Cytotoxicity assay	72 hrs	Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.11 μM.	28523103
MIAPaCa2	Cytotoxicity assay	72 hrs	Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.2 μM.	28523103
MDA-MB-231	Cytotoxicity assay	72 hrs	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.48 μM.	28523103
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay, IC50 = 0.46 μM.	28774427
HL60	Antiparasitic assay	72 hrs	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay, EC50 = 0.055 μM.	28803047
MOLT4	Antiparasitic assay	72 hrs	Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay, EC50 = 0.17 μM.	28803047
Vero	Cytotoxicity assay	72 hrs	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay, CC50 = 2.8 μM.	28803047
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay, IC50 = 0.45 μM.	29236492
HEK293T	Cytotoxicity assay	72 hrs	Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.42 μM.	29469575
HCT8	Antiparasitic assay	48 hrs	Antiparasitic activity against Cryptosporidium parvum BGF infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.55 μM.	29469575
HCT8	Antiparasitic assay	48 hrs	Antiparasitic activity against Cryptosporidium parvum SPL infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.6 μM.	29469575
HepG2	Cytotoxicity assay	72 hrs	Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.83 μM.	29469575
HCT8	Cytotoxicity assay		Cytotoxicity against human HCT8 cells, EC50 = 6.1 μM.	29469575
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay, IC50 = 0.46 μM.	29533611
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay, IC50 = 0.4 μM.	29969262
A549	Cytotoxicity assay	48 hrs	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM.	30418763
PC3	Cytotoxicity assay	48 hrs	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM.	30418763
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Chemische informatie, Opslag en Stabiliteit

Moleculair gewicht	544.43	Formule	C₂₂H₂₉N₇O₅.2HCl	Opslag (Vanaf de ontvangstdatum)	3 jaar-20°Cpoeder
CAS-nr.	58-58-2	SDF downloaden		Opslag van stamoplossingen	1 jaar-80°Cin oplosmiddel 1 maand-20°Cin oplosmiddel
Synoniemen	CL13900 2HCl			Smiles	CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl

Oplosbaarheid

In vitro
Batch:

DMSO : 100 mg/mL (183.67 mM)
(Met vocht verontreinigde DMSO kan de oplosbaarheid verminderen. Gebruik verse, watervrije DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble

Molariteitscalculator

Massa	Concentratie	Volume	Moleculair gewicht
=	×	×

Verdunningscalculator Moleculair gewicht calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formuleringscalculator (Heldere oplossing)

Stap 1: Voer de onderstaande informatie in (Aanbevolen: Een extra dier voor het geval van verlies tijdens het experiment)

Dosering: mg/kg Gemiddeld gewicht van dieren: g Doseervolume per dier:μL Aantal dieren:

Stap 2: Voer de in vivo formulering in (Dit is alleen de calculator, geen formulering. Neem eerst contact met ons op als er geen in vivo formulering is in het gedeelte Oplosbaarheid.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Berekeningsresultaten:

Werkconcentratie: mg/ml;

Methode voor het bereiden van DMSO-mastervloeistof: mg geneesmiddel vooraf opgelost in μL DMSO ( Concentratie mastervloeistof mg/mL, Neem eerst contact met ons op als de concentratie de DMSO-oplosbaarheid van de partij geneesmiddel overschrijdt. )

Methode voor het bereiden van in vivo formulering: Neem μL DMSO mastervloeistof, voeg vervolgens toeμL PEG300, mengen en helder maken, voeg vervolgens toeμL Tween 80, mengen en helder maken, voeg vervolgens toe μL ddH₂O, mengen en helder maken.

Methode voor het bereiden van in vivo formulering: Neem μL DMSO mastervloeistof, voeg vervolgens toe μL Maïsolie, mengen en helder maken.

Opmerking: 1. Zorg ervoor dat de vloeistof helder is voordat u het volgende oplosmiddel toevoegt.
2. Zorg ervoor dat u het/de oplosmiddel(en) in de juiste volgorde toevoegt. U moet ervoor zorgen dat de verkregen oplossing, bij de vorige toevoeging, een heldere oplossing is voordat u verdergaat met het toevoegen van het volgende oplosmiddel. Fysische methoden zoals vortexen, echografie of een warmwaterbad kunnen worden gebruikt om het oplossen te bevorderen.

Werkingsmechanisme

In vitro	Het antibioticum puromycin wordt geproduceerd door de actinomycete, Streptomyces alboniger, en is gebruikt als hulpmiddel voor het bestuderen van eiwitsynthese in een aantal systemen. Puromycin kan worden gebruikt voor de selectie van recombinante cellen uit niet-gekweekte cellen.
In vivo	Puromycin (aminonucleoside) remt de eiwitsynthese, wat leidt tot proteïnurie & glomerulosclerose/nefrose.
Referenties	[1] https://pubmed.ncbi.nlm.nih.gov/14211122/ [2] https://pubmed.ncbi.nlm.nih.gov/15385422/ [3] https://pubmed.ncbi.nlm.nih.gov/28971619/ [4] https://pubmed.ncbi.nlm.nih.gov/3953770/ [5] https://pubmed.ncbi.nlm.nih.gov/32435426/ [6] https://pubmed.ncbi.nlm.nih.gov/11397778/

Toepassingen

Methoden	Biomarkers	Afbeeldingen	PMID
Western blot	Cyclin D1 / CDK4 PARP / Bcl-xL / Bcl-2 / p-Akt / Akt p53 / p21 / c-Myc		31022952
Growth inhibition assay	Cell viability		31022952

Technische ondersteuning

Gebruiksaanwijzing

Tel: +1-832-582-8158 Ext:3

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Puromycin Dihydrochloride Eiwitsyntheseremmer

Chemische structuur

Moleculair gewicht: 544.43